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pRY121 plasmid in <em>E. coli</em>
pRY121 plasmid in <em>E. coli</em>
規(guī)格:
貨期:
編號(hào):B162052
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pRY121 plasmid in E. coli
商品貨號(hào) B162052
Designations pRY121 plasmid in E. coli
Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors JM Becker
Vector Total size of construct (kb): 11.50
Markers: ampR, lacZ+, URA3
Excise insert: SalI/BamHI

Vector: pLG67OZ
Vector type: plasmid
Vector size (kb):
Markers:
ampR
Insert Insert size (kb): 0.7
Gene: Galactose promoter region
Source: Saccharomyces cerevisiae
Insert ends: SalI/BamHI
Insert Size (kb) 0.700
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Frozen glycerol stock of Escherichia coli GC10 containing the plasmid.
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.2(doublet), 2.8, 2.2; HindIII--6.2, 2.15, 1.45, 1.05; SalI--11.5.
Contains S. cerevisiae GAL1 promoter region (from about 1 kb upstream of the GAL1 transcription start site to amino acid 33) fused to the bacterial lacZ gene.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

West RW Jr., et al. Saccharomyces cerevisiae GAL1-GAL10 divergent promoter region: location and function of the upstream activating sequence UASG. Mol. Cell. Biol. 4: 2467-2478, 1984. PubMed: 6392852

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