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Dengue virus type 1
Dengue virus type 1
規(guī)格:
貨期:
編號:B162832
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 Dengue virus type 1
商品貨號 B162832
Classification Flaviviridae, Flavivirus
Agent Dengue virus type 1
Strain Hawaii
Applications Vector-borne disease research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL per vial
Storage Conditions -70°C or colder
Images VR-1856 images
Comments Derived by adaptation of mouse-prepared product ATCC® VR-1254™ to Vero cells (ATCC® CCL-81™). ATCC® VR-1254™ and ATCC® VR-1856™ have not been compared for sequence or infectivity in tissue culture and in vivo.
Effect on Host
CPE, refractile rounding and sloughing
Recommended Host
Vero cells (ATCC® CCL-81™)
Growth Conditions

Temperature: 33°C

Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.02). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.

Incubation: 7-15 days at 33°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.

Name of Depositor R Selvarangan, Children’s Mercy Hospital
Source Pooled serum from 6 patients, Hawaii, 1944
References

Sabin AB. . Science 101: 640, 1945.

Sabin AB. Viral and Rickettsial Infections of Man. Philadelphia: JB Lippincott Co; 1959.

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
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