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IGEL a2 [15.3]
IGEL a2 [15.3]
規(guī)格:
貨期:
編號(hào):B164834
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) IGEL a2 [15.3]
商品貨號(hào) B164834
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor temperature
Genes Expressed
immunoglobulin; monoclonal antibody; against 2,4,6-trinitrophenyl (TNP)
Cellular Products
immunoglobulin; monoclonal antibody; against 2,4,6-trinitrophenyl (TNP)
Comments
Animals were immunized with TNP-Asc.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. Do not allow cell concentration to exceed 2 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37.0°C
Isotype IgE (Iga haplotype)
Name of Depositor M Wabl
Deposited As mouse (B cell); mouse (myeloma)
References

Rudolph AK, et al. Thirteen hybridomas secreting hapten-specific immunoglobulin E from mice with Iga or Igb heavy chain haplotype. Eur. J. Immunol. 11: 527-529, 1981. PubMed: 6790293

Cross References

Nucleotide (GenBank) : X65774 M.musculus DNA for IgE antibody light chain (VJ).

Nucleotide (GenBank) : X65772 M.musculus DNA for IgE antibody heavy chain (VDJ).

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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