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ILB1-H6 [ILB1-H6A1B]
ILB1-H6 [ILB1-H6A1B]
規(guī)格:
貨期:
編號(hào):B164855
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 ILB1-H6 [ILB1-H6A1B]
商品貨號(hào) B164855
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts specifically with IL-1 beta and does not react with IL-1 alpha or with acidic or basic fibroblast growth factor.
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Animals were immunized with purified recombinant human IL-1 beta (17500 dalton form).
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human interleukin 1 beta (interleukin-1 beta, IL-1 beta)
Cellular Products
immunoglobulin; monoclonal antibody; against human interleukin 1 beta (interleukin-1 beta, IL-1 beta)
Tumorigenic Yes
Effects
Yes, forms ascites in BALB/c mice
Comments
Animals were immunized with purified recombinant human IL-1 beta (17500 dalton form).
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts specifically with IL-1 beta and does not react with IL-1 alpha or with acidic or basic fibroblast growth factor.
It recognizes an epitope distinct from that bound by ILB1-H21 and ILB1-H67 (ATCC HB-10220 and ATCC HB-10222) and that may overlap the epitope bound by ILB1-H34 (ATCC HB-10221).
The affinity constant of ILB1-H6 was measured to be 0.78 X 10 exp10 liter/mol.
Complete Growth Medium The base medium for this cell line is HB101 medium (from Irvine Scientific). To make the complete growth medium, add fetal bovine serum to a final concentration of 7% Alternately, cells may be grown on ATCC Hybri-Care Medium Catalog No. 46-X as the base medium. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. It must be supplemented with 1.5 g/L sodium bicarbonate.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium, 90%; DMSO, 10%
Isotype IgG1; kappa light chain
Name of Depositor Syntex (USA), Inc.
Deposited As mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Allison AC, et al. Monoclonal antibodies for interleukin-1.beta. US Patent 4,935,343 dated Jun 19 1990

Kenney JS, et al. Monoclonal antibodies to human recombinant interleukin 1 (IL 1)beta: quantitation of IL 1 beta and inhibition of biological activity. J. Immunol. 138: 4236-4242, 1987. PubMed: 2438338

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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