| 產(chǎn)品名稱(chēng) |
LMH |
| 商品貨號(hào) |
B164992 |
| Organism |
Gallus gallus, chicken |
| Tissue |
liver |
| Cell Type |
chemically induced |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
hepatocellular carcinoma |
| Strain |
Leghorn |
| Applications |
transfection host |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
triploid; modal number = 116; six marker chromosomes |
| Derivation |
LMH is a primary hepatocellular carcinoma epithelial cell line established in 1981 by Tomoyuki Kitagawa at the Cancer Institute, Kami-Ikebukuro, Toshima-ku, Tokyo, Japan. |
| Clinical Data |
Tumorous nodules were induced in the liver of a male leghorn chicken by long term treatment with diethylnitrosamine. |
| Receptor Expression |
estrogen receptor, positive |
| Tumorigenic |
Yes |
| Effects |
Yes, forms tumors in nude mice |
| Comments |
Tumorous nodules were induced in the liver of a male leghorn chicken by long term treatment with diethylnitrosamine. The morphology of the cells is dendritic like. The cells express glucose-6-phosphatase and weak canalicular ATPase activity. The cells form tumors in athymic nude mice. LMH cells contain a low level of functional estrogen receptor and are inducible for the expression of the liver specific apolipoprotein II (apoII) gene. The cell line is useful for transfection studies. NOTE: tissue culture vessels used to propagate this line must be precoated with 0.1% gelatin. To prepare the vessels, flood them with gelatin solution, refrigerate for 5 to 10 minutes and remove the liquid. Vessels prepared in this fashion may be used immediately. |
| Complete Growth Medium |
Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%
|
| Subculturing |
Remove medium, rinse two times with cold 0.25% trypsin, 0.03% EDTA solution.
Allow the flasks to sit at room temperature (or incubate at 37°C) until the cells detach.
Add fresh culture medium, aspirate, and dispense into new culture flasks.
Flasks must be coated with 0.1% gelatin.
To prepare the vessels, flood them with gelatin solution, refrigerate for 5 to 10 minutes and remove the liquid.
Vessels prepared in this fashion may be used immediately.
Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Name of Depositor |
T Kitagawa, DL Williams |
| Deposited As |
Gallus gallus |
| Year of Origin |
1981 |
| References |
Binder R, et al. Expression of endogenous and transfected apolipoprotein II and vitellogenin II genes in an estrogen responsive chicken liver cell line. Mol. Endocrinol. 4: 201-208, 1990. PubMed: 2330000
Kawaguchi T, et al. Establishment and characterization of a chicken hepatocellular carcinoma cell line, LMH. Cancer Res. 47: 4460-4463, 1987. PubMed: 3607775
Sensel MG, et al. Reactivation of apolipoprotein II gene transcription by cycloheximide reveals two steps in the deactivation of estrogen receptor-mediated transcription. Mol. Cell. Biol. 14: 1733-1742, 1994. PubMed: 8114707
|
