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NCI-BL2009 [BL2009]
NCI-BL2009 [BL2009]
規(guī)格:
貨期:
編號(hào):B165237
品牌:Mingzhoubio

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產(chǎn)品名稱 NCI-BL2009 [BL2009]
商品貨號(hào) B165237
Organism Homo sapiens, human
Tissue peripheral blood
Cell Type B lymphoblast; Epstein-Barr virus (EBV) transformed
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2  [Cells Contain Epstein Barr Virus (EBV) a Herpesvirus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease normal
Age 68 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Comments

A lung adenocarcinoma cell line from this patient is available as ATCC CRL-5911.

ATCC confirmed this cell line is positive for the presence of Epstein-Barr viral DNA sequences via PCR.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 1 x 106 viable cells/mL. Maintain cell density between 106 and 3 x 106 cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 10,12
D13S317: 12
D16S539: 12
D5S818: 13
D7S820: 9,11
THO1: 9.3
TPOX: 8
vWA: 14,16
Name of Depositor AF Gazdar, JD Minna
Deposited As Homo sapiens
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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