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NCI-BL2122 [BL2122]
規(guī)格:
貨期:
編號:B165243
品牌:Mingzhoubio

標準菌株
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產品名稱 NCI-BL2122 [BL2122]
商品貨號 B165243
Organism Homo sapiens, human
Tissue peripheral blood
Cell Type B lymphoblast; Epstein-Barr virus (EBV) transformed
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 [Cells contain Epstein-Barr Virus (Herpesvirus)]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease Adenocarcinoma
Age 46 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Derivation

NCI-H2122 was derived from pleural effusion from a 46 year old White female with adenocarcinoma of the lung.

NCI-BL2122 BL is a B lymphoblastoid cell line initiated by Epstein-Barr virus (EBV) transformation of peripheral blood lymphocytes obtained from the same patient as NCI-H2122.
Clinical Data
46 years
Caucasian
female
Comments
The tumor cell line from this patient is available as ATCC CRL-5985.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 to 7 x 105 viable cells/mL. A maximum of 1 to 2 x 106 cells/mL is obtainable. Cultures grow as floating aggregates of large cell clusters. Add medium as cell density increases. Double volume approximately every 6 to 7 days.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 9,10
D16S539: 9,12
D5S818: 11,12
D7S820: 8,10
THO1: 7,9.3
TPOX: 9
vWA: 17
Name of Depositor AF Gazdar, JD Minna
Deposited As Homo sapiens
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
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沈經理 19548299266 2662369050
李經理 13626845108 972239479
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