国产91亚洲福利精品一区二区,国产综合成人久久大片91,国产成人精品久久综合,久久久91精品国产一区二区三区,91福利国产在线在线播放,91精品国产高清久久久久久91,91精品国产福利在线观看麻豆,国产免费一区二区三区在线观看

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > NCI-H2126 [H2126]
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
NCI-H2126 [H2126]
NCI-H2126 [H2126]
規(guī)格:
貨期:
編號:B165317
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 NCI-H2126 [H2126]
商品貨號 B165317
Organism Homo sapiens, human
Tissue lung; derived from metastatic site: pleural effusion
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease adenocarcinoma; non-small cell lung cancer
Age 65 years adult
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype Modal number = 79; range = 71 to 83
This is a hypertriploid human cell line with the modal chromosome number of 79, occurring in 20% of cells. The rate of cells with a higher ploidy was 5.5%. Karyotypes were very complex. There were over 22 marker chromosomes commonly present in most cells, many with complex structural rearrangements. Among these markers were: double copies for t(10qter--10q11.2::?::13C--13qter) and der(9)t(3;9) (p12;p22?), and one copy each for del (1) (p22) and i (iq). There were two normal X chromosomes per cell. Normal chromosomes Y, N1, N5, N14, and N15 were not found. Chromosomes N20 and N22 generally had four or more copies per cell.
Clinical Data
65 years adult
Caucasian
male
HeLa Markers N
Tumorigenic Yes
Effects
Yes, in nude mice
Comments
An EBV-transformed lymphoblastoid cell line from the same patient is available as ATCC CCL-256.1.
Complete Growth Medium HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1.  Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which   contains trypsin inhibitor.
  2. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell   layer is  dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  3. Add 6.0 to 8.0 mL of complete growth medium and aspirate  cells by gently pipetting. 
  4. Add appropriate aliquots of the cell suspension to new culture  vessels. An inoculum of 6 x 103 to 8 x 103 viable cells/cm2 is recommended.
  5. Or use Subcultivation Ratio: 1:2 to 1:4
  6. Incubate cultures at 37°C. Subculture when reaching a cell concentration between  8 x 104 and 1 x 105 cells/cm2.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11
D13S317: 12,14
D16S539: 12
D5S818: 11
D7S820: 8,9
THO1: 7,9.3
TPOX: 8
vWA: 17
Isoenzymes
AK-1, 1
ES-D, 1-2
G6PD, B
GLO-I, 2
Me-2, 0
PGM1, 1-2
PGM3, 2
Population Doubling Time about 41 hours
Name of Depositor AF Gazdar, JD Minna
Deposited As Homo sapiens
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
莱芜市| 三亚市| 南平市| 乌拉特后旗| 汤阴县| 个旧市| 志丹县| 耿马| 巴彦淖尔市| 东丰县| 旌德县| 正阳县| 崇左市| 永定县| 米泉市| 乐至县| 南溪县| 台东县| 同江市| 徐水县| 祁阳县| 大洼县| 九龙县| 米易县| 昌宁县| 永兴县| 陆丰市| 澜沧| 吉隆县| 浮梁县| 彭泽县| 和田市| 溧阳市| 扶绥县| 连江县| 广德县| 晋宁县| 甘谷县| 仁化县| 葵青区| 汉川市|