国产91亚洲福利精品一区二区,国产综合成人久久大片91,国产成人精品久久综合,久久久91精品国产一区二区三区,91福利国产在线在线播放,91精品国产高清久久久久久91,91精品国产福利在线观看麻豆,国产免费一区二区三区在线观看

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > S49 (Thy-1-a)
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
S49 (Thy-1-a)
S49 (Thy-1-a)
規(guī)格:
貨期:
編號:B165648
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 S49 (Thy-1-a)
商品貨號 B165648
Organism Mus musculus, mouse
Tissue lymphoma
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease lymphoma
Strain BALB/c
Applications
transfection host
Antigen Expression
H-2d; TL
Genes Expressed
H-2d; TL
Comments
This variant of S49.1TB.2 (ATCC TIB-30) does not express Thy-1.2 antigen.
The line was selected by 5 cycles of immunoselection using anti Thy-1 antiserum and complement.
This cell line is a Class A Thy-1- mutant.
The defect affects the biosysnthesis and/or assembly of the glycophospholipid membrane anchor.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation Freeze medium: Culture medium 95%; DMSO, 5%.
     Cell culture tested DMSO is available as ATCC 4-X
Storage temperature: liquid nitrogen vapor phase
Name of Depositor R Hyman
Deposited As Mus musculus
References

Hyman R. Studies on surface antigen variants. Isolation of two complementary variants for Thy 1.2. J. Natl. Cancer Inst. 50: 415-422, 1973. PubMed: 4702114

Ralph P, et al. Independence of theta and TL surface antigens and killing by thymidine, cortisol, phytohemagglutinin, and cyclic AMP in a murine lymphoma. Biochem. Biophys. Res. Commun. 55: 1085-1091, 1973. PubMed: 4358928

Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579

Trowbridge IS, et al. The synthesis and properties of T25 blycoprotein in Thy-1-negative mutant lymphoma cells. Cell 14: 21-32, 1978. PubMed: 667934

Conzelmann A, et al. Anchoring of membrane proteins via phosphatidylinositol is deficient in two classes of Thy-1 negative mutant lymphoma cells. EMBO J. 5: 3291-3296, 1986. PubMed: 2880714

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
青神县| 宝鸡市| 桐庐县| 平定县| 英吉沙县| 克拉玛依市| 十堰市| 沙洋县| 安泽县| 镶黄旗| 淮北市| 清流县| 同仁县| 平谷区| 洛隆县| 锦州市| 嘉兴市| 屯昌县| 竹北市| 同心县| 枣强县| 东平县| 当雄县| 灵台县| 浏阳市| 新民市| 乐清市| 昆山市| 湖南省| 福清市| 峨山| 海盐县| 海南省| 花莲县| 翼城县| 曲周县| 房山区| 攀枝花市| 泸水县| 忻城县| 梓潼县|