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SML, clone 4D8
SML, clone 4D8
規(guī)格:
貨期:
編號(hào):B165731
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 SML, clone 4D8
商品貨號(hào) B165731
Organism Saimiri boliviensis boliviensis, monkey, bolivian squirrel
Cell Type B lymphoblast; Epstein-Barr virus (EBV) transforme
Product Format frozen
Morphology lymphoblast
Culture Properties The cells grow in loose clumps., suspension
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Gender male
Applications
SML, clone 4D8 is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from a male squirrel monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).
Storage Conditions liquid nitrogen vapor phase
Karyotype The SML, clone 4D8 cells exhibit a karyotype consistent with the subspecies Saimiri boliviensis boliviensis; the chromosomal count was 44 with an XY sex chromosome constitution., G-banding revealed a pattern of the Bolivian karyomorph for the subspecies Saimiri boliviensis boliviensis with a submetacentric chromosomal pair 15 and an acrocentric chromosomal pair 16 resulting in six pairs of autosomal acrocentric chromosomes., The karyotype was heterozygous for the C-band polymorphism in the short arm of chromosome 14 and lacked the chromosome 5 C-band positive polymorphism found in Guyanese animals, Saimiri sciureus sciureus.
Derivation
SML, clone 4D8 is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from a male squirrel monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).
Clinical Data
SML, clone 4D8 is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from a male squirrel monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).
male
Antigen Expression
CD20 +
Genes Expressed
CD20 +
Comments
SML, clone 4D8 is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from a male squirrel monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).
The resulting cells were cloned by limiting dilution.
CITES Permit
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing Cultures can be maintained by addition or replacement of medium. When replacing media, centrifuge cells and resuspend cell pellet in fresh medium at 5 x 105 viable cells/mL. Maintain cultures at cell concentrations between 5 x 105 and 2 x 106 viable cells/mL.
Medium Renewal: Two to three times weekly
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Name of Depositor JG Scammell
Year of Origin 1995
References

Reynolds PD, et al. Cloning and expression of the glucocorticoid receptor from the squirrel monkey (Saimiri boliviensis boliviensis), a glucocorticoid-resistant primate. J. Clin. Endocrinol. Metab. 82: 465-472, 1997. PubMed: 9024238

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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