| 產(chǎn)品名稱 |
VERO 76 |
| 商品貨號 |
B165924 |
| Organism |
Cercopithecus aethiops |
| Tissue |
kidney |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Normal |
| Age |
adult |
| Storage Conditions |
liquid nitrogen vapor temperature |
| Derivation |
VERO 76 was derived in 1968 by K.M. Johnson and brought to Centers for Disease Control (CDC), Atlanta, GA. It is a derivative of the original VERO.
|
| Comments |
When infected with hemorrhagic fever viruses [Machupo (Bolivian), Junin (Argentinian), Lassa (African)], Marburg or Ebola viruses, these cells exhibited cytopathic effects. Plaques are also produced. VERO 76 has a lower saturation density than the original VERO (ATCC CCL-81). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:8 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions |
Temperature: 37°C |
| Name of Depositor |
EM Earley |
| Deposited As |
Cercopithecus aethiops |
| Year of Origin |
1968 |
| References |
Earley EM, Johnson KMThe lineage of Vero, Vero 76 and its clone C1008 in the United StatesIn: Earley EM, Johnson KMVero cells: origin, properties and biomedical applicationsTokyoChiba Univ.pp. 26-29, 1988
Policastro PF, et al. Improved plaque assays for Rickettsia prowazekii in Vero76 cells. J. Clin. Microbiol. 34: 1944-1948, 1996. PubMed: 8818887
Schramm N, et al. Vesicles containing Chlamydia trachomatis serovar L2 remain above pH 6 within HEC-1B cells. Infect. Immun. 64: 1208-1214, 1996. PubMed: 8606080
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